Small subunit ribosome biogenesis
The small ribosomal subunit is responsible for decoding of genetic information. In eukaryotes, small subunit assembly involves the initial formation of a large nucleolar particle termed the small subunit processome. Within this complex, the immature small ribosomal subunit is chaperoned by more than 50 ribosome assembly factors. We have analyzed the formation of this particle as a function of transcription (Chaker-Margot et al. 2015), determined the roles of multi-protein complexes within it (Hunziker et al. 2016) and determined its structure by cryo-EM (Chaker-Margot et al. 2017, Barandun et al. 2017). Emerging principles of these studies (Barandun et al. 2018) are the basis for several lines of research in the lab.
References
- Stage-specific assembly events of the 6-MDa small-subunit processome initiate eukaryotic ribosome biogenesis.
Chaker-Margot M, Hunziker M, Barandun J, Dill BD, Klinge S.
Nat Struct Mol Biol. 2015 Nov;22(11):920-3. doi: 10.1038/nsmb.3111 - UtpA and UtpB chaperone nascent pre-ribosomal RNA and U3 snoRNA to initiate eukaryotic ribosome assembly.
Hunziker M*, Barandun J*, Petfalski E, Tan D, Delan-Forino C, Molloy KR, Kim KH, Dunn-Davies H, Shi Y, Chaker-Margot M, Chait BT, Walz T, Tollervey D, Klinge S.
Nat Commun. 2016 Jun 29;7:12090. doi: 10.1038/ncomms12090. - The complete structure of the small-subunit processome.
Barandun J*, Chaker-Margot M*, Hunziker M*, Molloy KR, Chait BT, Klinge S.
Nat Struct Mol Biol. 2017 Nov;24(11):944-953. doi: 10.1038/nsmb.3472. Epub 2017 Sep 25. - Assembly and structure of the SSU processome-a nucleolar precursor of the small ribosomal subunit.
Barandun J*, Hunziker M*, Klinge S.
Curr Opin Struct Biol. 2018 Jan 31;49:85-93. doi: 10.1016/j.sbi.2018.01.008.